| abstract |
FIELD: biotechnology and genetic engineering.SUBSTANCE: inventions group relates to the field of biotechnology and genetic engineering. Described is a recombinant virus-like particle for inducing specific immunity against the severe acute respiratory syndrome virus SARS-CoV-2, containing recombinant E, M, N coronavirus proteins and one or more S proteins of various coronavirus strains, containing the nucleotide sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, which are obtained in a baculovirus insect expression system, while the virus-like particle mimics the SARS-virion CoV-2. Also disclosed is a method for producing a recombinant virus-like particle. And a recombinant baculovirus is claimed to produce a recombinant virus-like particle, comprising a gene selected from the group: a gene encoding a coronavirus E protein having the nucleotide sequence of SEQ ID NO:1, a gene encoding a coronavirus M protein having the nucleotide sequence of SEQ ID NO:2, a gene, encoding the coronavirus N protein having the nucleotide sequence of SEQ ID NO:3, a gene encoding the coronavirus S protein having the nucleotide sequence of SEQ ID NO:4, a gene encoding the coronavirus S protein having the nucleotide sequence of SEQ ID NO:5, a gene encoding the coronavirus S protein having the nucleotide sequence of SEQ ID NO:6; a gene encoding the coronavirus S protein having the nucleotide sequence of SEQ ID NO:7.EFFECT: inventions group provides for obtaining stable virus-like particles containing actual antigens of the SARS-CoV-2 coronavirus, which are capable of self-assembling in insect cells that mimic the coronavirus virion and are capable of inducing a specific immune response against the severe acute respiratory syndrome virus SARS-CoV-2.7 cl, 7 dwg, 4 ex |