http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2761266-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_34fa08f37a5571d671371ce3effab321 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-491 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-15 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-49 |
| filingDate | 2021-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01cd196e7d52af5009b43ecdf5859b51 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b14aa78ffaed5bb6d6685055200cff7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ffba6b7e3a447391f7bb167d40f3f0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77f663fd5ae8d887fd2b4fff14b8d20d |
| publicationDate | 2021-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2761266-C1 |
| titleOfInvention | Method for assessing suitability of synthetic polymers being developed for medical purposes |
| abstract | FIELD: medicine.SUBSTANCE: invention relates to medicine, namely biotechnology, immunology, implantology, and can be used to assess the suitability of synthetic polymers for medical purposes. A sample of peripheral venous blood is obtained from a volunteer, then it is centrifuged to obtain a population of mononuclear leukocytes. The resulting cell suspension is introduced into the wells of a round-bottom plate with samples of synthetic polymers placed in them in the form of a cylinder with a length of 3 mm and a diameter of 1 mm in the amount of 1 piece per well. The plate is incubated at 5% CO2for 72 hours at a temperature of 37°C. The number of mononuclear leukocytes and the concentration of anti-inflammatory cytokines IL-4 and IL-10 are determined, expressing the obtained indicators in the form of a stimulation index (SI), which is calculated by the formula: SI =SPP/SPG, having previously determined the specific production of each of the cytokines in the presence of a synthetic polymer (SPP, pg/k) and the specific production of each of the cytokines in the presence of glass (SPG, pg/kl) according to the formulas:SPP = P/NLs; SPG = G/NLg, whereP is the cytokine concentration in a sample with a synthetic polymer, pg/ml;NLsthe number of mononuclear leukocytes in a sample with a synthetic polymer, in 1 ml;G is the concentration of cytokine in a sample with a glass sample, pg/ml;NLgis the number of mononuclear leukocytes in a sample with glass, in 1 ml. The suitability of the use of synthetic polymers for medical purposes is evaluated. An SI value equal to or greater than 1 indicates the suitability of their use for medical purposes.EFFECT: method provides increased efficiency, reduced time and labor costs, the possibility of simultaneous verification of a significant number of samples, a comprehensive assessment of the proliferative and cytokine-producing activity of mononuclear cells in direct contact with the samples, making it possible to determine the direction of the immune response, justifying the choice of a promising material for medicine, by implementing a contact method for exposure of cells of the immune system, mononuclear leukocytes contained in human peripheral venous blood to synthetic polymer samples, with an assessment of their production of anti-inflammatory cytokinesin vitroaccording to the index of stimulation, which in a comparative analysis of the test results makes it possible to select the most promising samples.1 cl, 3 tbl, 3 ex |
| priorityDate | 2021-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.