abstract |
FIELD: biotechnology. n SUBSTANCE: invention relates to biotechnology and is an application of psicose-6-phosphate phosphatase for the production of D-psicose, where psicose-6-phosphate phosphatase contains A motif, represented by X a1 -X a2 -X a3 -DPLDG-X a4 or X a1 -X a2 -X a3 -DPIDG-X a4 , wherein X a1 is W, F, V, I or A, X a2 is I, F, V, A or absent, X a3 is V, I or L, and X a4 is T or S, and B motif, represented by Y a1 -D-Y a2 -W a1 -Y a3 -W a2 -Y a4 -W a3 , wherein Y a1 is W, Y, T, L or V, Y a2 is V, I, C, F or A, W a1 is AAG, AAS, SAG, APG, APF, AGG, APL or AGA, Y a3 is W, I, P, M, V, Y, F, R, L, T or S, W a2 is LLV, LIV, LLI, LII, ILI, FIA, ALV, IIA, VLV, VIL, TIG, NFC or PIF, Y a4 is E, R, S, T, L, K or P, and W a3 is EAGG, EGGG, EAKG, KAGG, AAGG, YVDG, EAGA or RLGV. n EFFECT: invention makes it possible to increase the yield of D-psicose. n 16 cl, 4 dwg, 1 tbl, 5 ex |