http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2720470-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5f220ff41ebe4c7f2e7da8ec7e7e9dca |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0062 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61F9-007 |
| filingDate | 2018-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5dad106e64ca30545e8212b6b01b1f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd5fa3fc3cb12fba9adc2749fb9ed31d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_364bd3b494ccfb6e09be22cd0d8d4b50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa44735f24c7855825cbfd632df90093 |
| publicationDate | 2020-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2720470-C1 |
| titleOfInvention | Method of producing transplant for treating limbal insufficiency |
| abstract | FIELD: biotechnology. n SUBSTANCE: invention refers to biotechnology, namely to obtaining a transplant for treating limbal insufficiency. Method involves mechanical cleaning of allogenic sclera, its soaking in 6 % hydrogen peroxide solution and maintenance for 4 hours (destruction of tissue pigments, lipid structures and disinfection), then washed with sterile distilled water and frozen at a temperature of -20 °C for 2 hours (which enables to destroy cell membranes). Then defrostation is soaked in water solution of ammonium hydroxide 10 % for 2 hours at temperature of 40 °C (which promotes washing out of lipids, but does not cause collagen denaturation). Then one performs washing with sterile physiological solution, soaking in 6 % hydrogen peroxide solution and holding for 6 hours. Then one performs washing with sterile normal saline, dividing into fragments 2 mm wide and 4 mm long and soaked in physiologic saline for 20 minutes. Fragments are placed in culture plates with 10 ml of culture medium DMEM/F12 with addition of 15 % of embryonal serum and added with 1 ml of concentrated cell suspension MMSC and progenitor cells of epithelium of cornea (0.5–0.6 × 10 6 cells/ml) and incubated for 5 days. n EFFECT: disclosed is a method for producing a transplant for treating limbal insufficiency. n 1 cl, 1 tbl, 2 ex |
| priorityDate | 2018-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.