http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2675930-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dbff901ee08b196c9a837bd6a96a836c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0665 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 2017-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0b9521360cac1189c7abe07ee1850e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_abb4e2f3e1db77b4cba098d8d62e5c3d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a8e9d2841fe65efccba35256379fed5e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c326dc66436dd744788845741c3ad06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6633f5f2c7509d0bd59d0cb7f9488156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c2b6fa00e45bd3b12d62e53f0a0509b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9a630ce6c6b4a3cf0baee25756efdfb |
| publicationDate | 2018-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2675930-C1 |
| titleOfInvention | Cell culture and biotransplant for bone tissue regeneration on its basis |
| abstract | FIELD: medicine; biotechnology. n SUBSTANCE: invention relates to the field of biotechnology, in particular to the production of multipotent mesenchymal stromal cells cultured as spheroids and induced in the angiogenic direction. Method includes isolating a donor tissue fragment containing multipotent mesenchymal stromal cells, transferring a donor tissue fragment to a medium containing culture medium, an amino acid and an antibiotic, mechanical grinding of a fragment of donor tissue with obtaining a homogenate, incubating the obtained homogenate in a solution of proteolytic enzymes at 37 °C until receiving the cell suspension. Next, add a nutrient medium containing culture medium, amino acid, antibiotic, serum; the cell suspension is centrifuged until the cells are completely precipitated, the supernatant is removed, the isolated cells are resuspended in a nutrient medium containing culture medium, an amino acid, an antibiotic, blood serum, cultivation of selected cells in monolayer culture at 37 °C in an environment of 5 % CO 2 at least 2 passages, monolayer disaggregation, cultivation of cells of at least 2 nd passage in non-adhesive conditions in concentration from 1 × 10 6 cells/ml to 5 × 10 6 cells/ml in a nutrient medium containing culture medium, amino acid, antibiotic, blood serum, angiogenesis induction factor to produce a cell culture containing multipotent mesenchymal stromal cells cultured as spheroids. n EFFECT: invention allows to obtain multipotent mesenchymal stromal cells cultured as spheroids and induced in the angiogenic direction. n 25 cl, 3 dwg, 3 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2747087-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021086222-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2744664-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2731314-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2802060-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2779742-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2750021-C1 |
| priorityDate | 2017-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 309.