http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2661105-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3d70184fbd9d12b8ef1954f40822a0c1 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 |
| filingDate | 2016-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adfa231a2a40faae04a514f137650595 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1186fe618324c349864df8b4c99ae60f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0f8c50ce41ac9dee67b48ef35a2bcd3 |
| publicationDate | 2018-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2661105-C2 |
| titleOfInvention | Method for producing spheroids of heparg cells in a medium without dimethyl sulphoxide |
| abstract | FIELD: biotechnology. n SUBSTANCE: invention relates to biotechnology, namely to production of spheroids of HepaRG cells expressing cytochromes P450 3A4, 2B6, 2C19, 2C9. Method comprises culturing HepaRG cells in a complete William's E culture medium supplemented with L-glutamine, 10 % fetal bovine serum, 5 mcg/ml recombinant human insulin, 5x10 -5 M hydrocortisone hemisuccinate, 1 % penicillin/streptomycin, in CO 2 -incubator at 37 °C, 95 % air, 5 % CO 2 and relative humidity of 98 %, induction. Method further includes transfer to 96-well plates with low adhesion and a U-shaped bottom to form spheroids and the subsequent incubation of the spheroids in serum-free William's E nutrient medium supplemented with L-glutamine, 1x Insulin-Transferrin-Selenium, 1 mcg/ml bovine serum albumin, 5x10 -5 M hydrocortisone hemisuccinate, 1x interchangeable amino acids, 1 % penicillin or streptomycin at 37 °C, 95 % air, 5 % CO 2 and a relative humidity of 98 %. n EFFECT: invention allows to obtain spheroids of HepaRG cells expressing cytochromes P450 3A4, 2B6, 2C19, 2C9, to study the metabolism and toxicity of pharmaceuticals. n 4 cl, 2 dwg, 2 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2747087-C1 |
| priorityDate | 2016-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 397.