| abstract |
FIELD: veterinary science. n SUBSTANCE: present invention relates to field of veterinary vaccines, in particular to vector vaccines for poultry based on recombinant nonpathogenic Marek's disease virus (npMDV). Invention also relates to methods and applications, including recombinant npMDV, expression cassette, infected host cell and a vaccine. Recombinant npMDV contains heterologous nucleic acid molecule, wherein said nucleic acid molecule contains from 5′-end to 3′-end in following order: a. core early 1 gene promoter of human cytomegalovirus (hCMV-IE1), where said promoter is presented in SEQ ID NO: 1, (b). protein gene fusion (F) Newcastle disease virus (NDV), c. terminator transcription, d. core chicken beta-actin gene promoter, where said promoter is presented in SEQ ID NO: 4, ie. viral protein gene 2 (VP2) virus infectious bursitis (IBDV) classic type. n EFFECT: vaccine based on recombinant npMDV, can be used for induction of protective immune response in poultry not only against Marek's disease, but also against Newcastle disease and infectious bursitis treatment. n 11 cl, 5 dwg, 3 tbl, 8 ex |