http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2233879-C1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-61 |
| filingDate | 2002-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2004-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_523ffc0b1ae23bba05d10c65eda504c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8dc0bea40e6c0dab0407f73bf75b1ccd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05398cea6dc347c249a13f69a382daed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cda7fcfb3d752858fc5e1bdc3bbe16a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44c2409a0a47b4ac6ecc4327229013d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bde544ce4a3cedea4c37a8dab3c68b64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0405e5a8763b8ccbb00fb092b9a1b9df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98b00ec6d0aa46cea3dd7519f467ede5 |
| publicationDate | 2004-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2233879-C1 |
| titleOfInvention | Recombinant plasmid dna pes1-6 encoding polypeptide somatotropin and strain escherichia coli bl21(de3)/pes1-6 as producer of recombinant somatotropin |
| abstract | FIELD: biotechnology, genetic engineering, molecular biology. n SUBSTANCE: invention can be used for preparing recombinant human growth hormone. Recombinant plasmid DNA pES1-6 encoding polypeptide with somatotropin amino acid sequence with molecular mass 3.66 MDa (5949 pair bases) consists of: Nde I/Xho I DNA fragment of plasmid pET22b(+) comprising transcription promoter and terminator of T7 RNA-polymerase, translation enhancer of phage T7 gene 10, β-lactamase gene and Nde I/Xho I DNA fragment comprising the sequence of artificial gene for recombinant somatotropin comprising: as genetic marker β-lactamase gene determining resistance of E. coli cells transformed with plasmid pSE1-6 to ampicillin; unique recognition sites for site-specific endonuclease restriction enzymes located at the following distance to the right of site Nde I : Xba I - 38 pair bases, Hpa I - 1332 pair bases, Pst I - 4065 pair bases, Pvu I - 4190 pair bases, Xho I - 5363 pair bases. Invention provides preparing the strain Escherichia coli BL21(DE3)/pES1-6 comprising recombinant plasmid DNA pES1-6 as a producer of recombinant somatotropin. Invention provides preparing recombinant somatotropin with high yield by the simplified technology. n EFFECT: improved preparing method, valuable biological properties of plasmid DNA. n 3 cl, 2 dwg, 2 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019070162-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2465315-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-035207-B1 |
| priorityDate | 2002-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 219.