| abstract |
The present invention relates to a process for the production of butanediol from ethanol and its various applications. The method of producing butanediol in ethanol of the present invention is a method of designing an artificial synthetic pathway so that NOX, EtDH, FLS, and BDH proteins and their mutant proteins are displayed as multi-step catalytic activities as enzymes, and using a cell-free catalyst method Respectively. The method of producing butanediol of the present invention does not require cell growth as compared with conventional microbial fermentation methods, and can easily produce a short synthesis route, a fast reaction rate, a high yield and productivity, and a desired reaction condition, have. In addition, the protein can be fixed to nanoparticles and can be reused in many ways, and is effective for producing butanediol, which is economical. Therefore, it can be usefully used in related industries in which butanediol is required as an active ingredient. |