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filingDate 2017-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c35d28f708c11bd3dbdb0f97f8ef71db
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publicationDate 2018-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20180135597-A
titleOfInvention Preparation method of hydrogel based on decellularized tissue using supercritical fluid-organic solvent system
abstract The present invention relates to a method for preparing a hydrogel based on deagglomerated tissue using a supercritical fluid-organic solvent system, and more particularly, to a method for producing a hydrogel by using a supercritical fluid-organic solvent system, ; (b) washing said degummed tissue; (c) preparing a de-saturated tissue solution by mixing the washed de-saturated tissue with pepsin and an acidic solution; (d) treating the de-saturated tissue solution with a basic solution to a pH of 5.5 to 7.8; And (e) allowing the de-saturated tissue solution to stand at 30 to 40 DEG C to prepare a de-saturated hydrogel which maximizes the preservability of various tissue-derived proteins, growth factors, and cytokines, The present invention relates to a technique applied to a tissue engineering material having improved productivity and tissue regeneration ability.
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