http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20160101806-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_259697bb7506be6263dc8926165c2806 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2015-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f13c91ae6b481017d315c140bd1dde5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef157b48d0793c238c04ad4f82f32781 |
| publicationDate | 2016-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20160101806-A |
| titleOfInvention | DNA structure for selective expression of the therapeutic gene |
| abstract | The present invention relates to a DNA construct comprising a target sequence of i) a therapeutic gene and ii) a target sequence of a passenger strand of a miRNA, wherein the miRNA is present in normal cells, is absent or decreased in the disease cells, wherein the target sequence of the passenger strand of the miRNA inhibits the expression of the therapeutic gene in normal cells, and a pharmaceutical preparation containing the DNA construct as an active ingredient. Using the present invention, the target sequence of the passenger strand of a specific miRNA that is expressed in normal cells but not in abnormal cells or diseased cells or whose expression is reduced is placed in the corresponding region of the 3 'untranslated region (UTR) of the therapeutic gene The therapeutic gene can be expressed only in abnormal cells, so that the therapeutic gene can function only in abnormal cells. Thus, there is an advantage that the disease can be treated by expressing the therapeutic gene only in the diseased tissue. The present invention also relates to a DNA construct comprising i) a reporter gene and ii) a target sequence of a passenger strand of a miRNA, wherein the miRNA is present in normal cells and is absent or decreased in expression in disease cells A target sequence of a passenger strand of the miRNA comprises a DNA construct which inhibits the expression of a reporter gene in normal cells as an active ingredient and a DNA construct comprising the reporter gene into a biological sample; And detecting the reporter protein in the sample. The present invention also relates to a method for providing information for diagnosis of a disease. According to this, by including the reporter gene in the DNA construct, the reporter gene is not active in the normal cell, but the reporter gene is activated only in the abnormal cell or disease cell, so that the disease can be diagnosed easily. |
| priorityDate | 2015-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1411.