http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20140147604-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_259045c6eba6e2c5d338b84e0a88b571 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2013-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce7971a8f4d3f10415896caa00eeda7b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07327c33ce52dc8564b122a8c1039f53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a85ee27f4ebe58ce61682b57a886827 |
| publicationDate | 2014-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20140147604-A |
| titleOfInvention | Method of Telomerase Activity Assay using Fluorescence |
| abstract | The present invention relates to a method for measuring telomerase activity using fluorescence, and more particularly, to a method for measuring telomerase activity using fluorescence, comprising the steps of: (a) reacting a biological sample and a TS primer in the presence of dNTP to elongate TTAGGG repeatedly at the 3 'end of the TS primer; (b) treating the elongated TS primer with a cation to form a G-quadruplex, and then reacting with a guanine-quadrupole structure formed by adding an intercalating fluorescent material; And (c) measuring fluorescence after the reaction. The method according to the present invention can be used for a telomerase activity assay kit because the telomerase activity can be measured rapidly and sensitively without a PCR process. In addition, it can be easily applied to high-speed search systems (HTS) and automation devices because the fluorescence expression is sensitive to the presence of the telomerase inhibitor and the TS primer does not need to be fixed on another support, And can be used for cancer diagnosis and initial screening of a telomerase inhibitor as an anticancer agent. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104975103-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108956990-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109182456-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111269960-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108956990-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109182456-A |
| priorityDate | 2013-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 101.