http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109182456-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 |
| filingDate | 2018-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109182456-B |
| titleOfInvention | A method for detection of telomerase activity in urine based on hybridization chain reaction and dynamic light scattering |
| abstract | The invention discloses a method for detecting telomerase activity in urine based on hybridization chain reaction (HCR) and dynamic light scattering (DLS). The method is that in the presence of active telomerase, the extension product of its substrate will trigger the HCR reaction of probes H1 and H2 to form a long, nicked double-stranded DNA product. The HCR product can then be hybridized with DNA-modified gold nanoparticles (DNA‑AuNP) probes, resulting in the aggregation of gold nanoparticles. The change of the dynamic light scattering signal (particle size) of gold nanoparticles was measured to achieve a highly sensitive quantitative analysis of telomerase activity. The method has the advantages of simple operation, low detection cost, few samples required, high sensitivity and good specificity. At the same time, we applied the method to the detection of telomerase activity in the urine of various cancer patients, and the results showed that the present invention has a specific response to bladder cancer, and provides a new way for non-invasive diagnosis of bladder cancer. |
| priorityDate | 2018-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.