| abstract |
The present invention relates to a method for analyzing the interaction between proteins in a cell, and more specifically, a polynucleotide encoding a first fusion protein consisting of a bait protein, a first fluorescent protein, and a cellulose-binding domain (CBD) protein. Providing a cell comprising a first construct comprising a second construct comprising a polynucleotide encoding a second fusion protein consisting of a target protein and a second fluorescent protein, and expressing the fusion protein Forming an inclusion body in the cell, and then analyzing the interaction between proteins in the cell by measuring the fluorescent signal of the fluorescent protein, and separating the target protein that binds to the target protein using the cells including the structures. It relates to a kit for analyzing the interaction between the method, the cell, and the protein comprising the construct. |