| abstract |
In the present invention, PERV (Porcine Endogenous Retrovirus) EnvC is negative, GGTA1 (α1,3-galactosyltransferase), CMAH (CMP-N-acetylneuraminic acid hydroxylase), iGb3s (Isoglobotrihexosylceramide synthase) and β4GalNT2 (Beta-1,4-N- Acetyl-Galactosaminyl Transferase2) is knocked out, and it relates to a transgenic cloned pig for xenotransplantation expressing human CD46 and Thrombomodulin (TBM) genes, and a method for producing the same. Transgenic cloned pigs according to the present invention do not cause metastasis of porcine endogenous retrovirus that occurs in xenotransplantation, but are superacute and antigen-antibody-mediated immune rejection, immune rejection by blood coagulation, and complement activity. Since it can overcome immune rejection, it can be usefully utilized as a donor animal for transplantation of heterogeneous organs and cells. |