http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101380728-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-85 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-745 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-745 |
| filingDate | 2011-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101380728-B1 |
| titleOfInvention | Mass production method of 7 factors of human blood coagulation |
| abstract | The present invention relates to a method for mass-producing human blood coagulation factor 7, and more particularly, to a method for mass production of human blood coagulation factor 7, comprising the steps of: a) preparing a dihydrofolate reductase promoter from which at least one CCGCCC has been removed in a GC- A base sequence encoding a nucleotide sequence and dihydrofolate reductase (DHFR) operably linked thereto; And ii) a nucleotide sequence encoding a cytomegalovirus (CMV) initial gene promoter and a nucleotide sequence encoding human blood coagulation factor 7 operably linked thereto. b) transforming the animal cell line with the expression vector of step a); c) culturing the transformed animal cell line of step b) in the presence of a dihydrofolate reductase inhibitor to select a cell line expressing human blood coagulation factor 7 with high efficiency; And d) adding sodium butyrate to the selected animal cell line of step c), and a method for mass-producing human blood coagulation factor 7 and a cell line for mass-producing human blood coagulation factor 7. The present invention can express human hemagglutinin factor 7 in high efficiency and in a large amount by using a vector in which the GC-rich repeat sequence of the DHFR promoter region is deleted, and thus can be usefully applied to the preparation of a hemophilia therapeutic agent. |
| priorityDate | 2010-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 161.