http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100880509-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2006-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2009-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100880509-B1 |
| titleOfInvention | Novel vectors, expression cell lines, and methods for producing recombinant proteins using the same for mass production of recombinant proteins |
| abstract | The present invention provides a high expression induction cassette, the expression induction cassette, and a selective expression comprising a promoter of dihydrofolate reductase from which all or part of a GC-rich repeat sequence has been removed, which can improve gene amplification technology more efficiently. The present invention relates to an expression vector further comprising a gene encoding a recombinant protein of interest, an animal cell line transformed by these expression vectors, and a method for mass production and purification of the recombinant protein by culturing the transformed animal cell line. According to the present invention, a short period of use of a low concentration of dihydrofolate reductase inhibitor can shorten the development period of a cell line capable of producing a high concentration of the recombinant protein of interest, thereby enabling the production of a more efficient recombinant protein. n n Dihydrofolate reductase, GC-rich repeat, recombinant protein, high expression |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9243237-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101380728-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11254724-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013051900-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3417881-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3348274-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015030479-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012057527-A2 |
| priorityDate | 2006-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 295.