| abstract |
Antibodies are engineered by replacing one or more amino acids of the parent antibody with non-crosslinked, highly reactive cysteine amino acids. Antibody fragments are also engineered with one or more cysteine amino acids to form cysteine engineered antibody fragments (ThioFab). Methods of designing, preparing, screening and selecting cysteine engineered antibodies are provided. Cysteine engineered antibodies (Ab), optionally together with albumin-binding peptide (ABP) sequences, are conjugated with one or more drug moieties (D) via a linker (L) to form a cysteine engineered antibody-drug conjugate of formula (I) do. Diagnostic and therapeutic uses for cysteine engineered antibody drug compounds and compositions are disclosed.n n n <Formula I>n n n n n n n n In formula, p is 1-4.n n n Antibody-drug conjugate compounds, tumor-associated antigens, cysteine engineered antibodies, cancer |