http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100475211-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-146 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2002-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2005-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2005-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100475211-B1 |
| titleOfInvention | Nucleic acid molecules encoding hyperactive mutant phytochromes and uses thereof |
| abstract | The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which the DNA sequence encoding the "active site" amino acid residue is mutated to produce hypersensitive phytochromes. In particular, the serine / threonine residues are not only at the hinge between the N- and C-terminal domains, but also for (a) Pr / Pfr-dependent phosphorylation and (b) dephosphorylation by phytochrome phosphatase (PP2A). N-terminal seri / threonine clusters of phytochromes (ie serine-598 and seri-7 in oat phytochrome A) were also substituted by alanine. (c) Moreover, amino acid residues in the phytochrome chromophore pockets are mutated to cause a bathchromic shift of the Pr-absorbing bands of both the wild type and the above-described mutant phytochromes. Plants with deeply chromophorically shifted absorption spectra are expected to respond to canopy and shade conditions for growth and to have a greening response with greater efficiency for far-red light than wild type plants with normal absorption band maxima. This mutagenic modification confers hypersensitivity to far-red light reactive phytochrome A. Thus, the biological activity of the modified oat PHYA was characterized by hypersensitivity, characterized by its ability to reduce the interstitial elongation of adult plants compared to wild type PHYA. Overexpression of phytochrome phosphatase shows suppressed growth and slowed flowering with shorter intercutions and is consistent with the phenotype of the ser598ala mutant oat phytochrome. The invention also encompasses a plant having at least one or more cells expressing a modified PHYA, a vector consisting of at least a portion of the modified PHYA nucleic acid, and a method of using such a vector to produce a plant with reduced kidneys. |
| priorityDate | 2002-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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