http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100342437-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0602 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-075 |
| filingDate | 1999-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2002-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100342437-B1 |
| titleOfInvention | Method for enucleation of oocyte for producing a clone animal using somatic cell |
| abstract | The present invention relates to a method for denuclearization of mature eggs that can be used in the production method of cloned animals, the working dish having the mature eggs to be denuclearized on the operation plate, the fixed pipette on the left arm of the operating device, the pipette for cutting on the right side Mounting a; Moving the pipette up and down so that the first pole body is positioned at 12 o'clock of the fixed pipette, and holding the fixed pipette in close contact with the mature o'clock at 9 o'clock to apply hydraulic pressure to fix the egg; Penetrate the incision pipette at 1 o'clock, pass through the zona pellucida, and be careful not to injure the cytoplasm. Penetrate at 11 o'clock, apply positive pressure to the fixation pipette to separate mature eggs and remove the fixation pipette. After contacting the transparent zone of the monopole upper portion, rubbing the two pipettes to cut the transparent zone; And positioning the incision window vertically by rotating the incised egg, and then placing the fixing pipette at the bottom of the mature egg to prevent the mature egg from moving downward, and then pressing the incision pipette on the mature egg to include the first polar body. And denuclearizing by removing 10-30% of the cytoplasm.n n n In addition, using the holding pipette and the incision pipette to rotate the incision of the mature egg in the vertical direction at 3 o'clock, press the holding pipette and the incision pipette from above to remove 10-30% of the cytoplasm including the first polar body Denuclearization may be performed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100839172-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007013763-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009088189-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100891487-B1 |
| priorityDate | 1999-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 142.