http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2005090604-A1

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filingDate 2005-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2008-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-WO2005090604-A1
titleOfInvention Gene mutation detection method and gene mutation detection kit
abstract A solution containing a single-stranded target nucleic acid (10) having a target base (11) related to SNP and the like, and two types of single-stranded detection nucleic acids complementary to a partial sequence sandwiching the target base (11) ( The solution containing 20a) and (20b) is mixed, and the target nucleic acid (10) and the detection nucleic acid (20a) and (20b) are hybridized, thereby intentionally forming a site facing the target base (11). A gap site (21) is constructed. Then, a receptor molecule (30) exhibiting hydrogen bonding and fluorescence is inserted into this gap site (21) which is a hydrophobic field space. Then, the fluorescence intensity change of the receptor molecule (30) based on the difference of the target base (11) is detected, and single base substitution is detected.
priorityDate 2004-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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