| abstract |
An object of the present invention is to detect a single base substitution of a gene simply and quickly by minimizing the number of steps. SOLUTION: In a single-stranded nucleic acid for detection 20 to be hybridized with a single-stranded target nucleic acid 10 containing a target base 11 related to SNP, a base at a position corresponding to the target base 11 is previously removed, and abasic This is set as a part 21. Then, after the target nucleic acid 10 and the detection nucleic acid 20 are hybridized, a hydrophobic field space is intentionally constructed around the target base 11, and the hydrogen bond type receptor molecule 30 is inserted into the hydrophobic field space. . Thereafter, a change in the fluorescence intensity of the receptor molecule based on the difference in the target base is detected, and a single base substitution is detected. [Selection diagram] Fig. 1 |