http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S63214187-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_696ca0ae93d5cf5a686273601361781f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-54 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1987-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec1bc98dc4de1edf19f71a59ba34f2ae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7fb6e86195f19af81c19dfa287f88ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66f9a134a04e122d9f0584ec11476339 |
| publicationDate | 1988-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S63214187-A |
| titleOfInvention | Production of novel genetic dna and alkaline protease |
| abstract | PURPOSE:To efficiently obtain an alkaline protease useful as a cleaning assistant in mass-production, by cultivating a bacterium of the genus Bacillus transformed with a plasmid containing a specific genetic DNA. CONSTITUTION:A bacterium Bacillus licheniformis (BR) is subjected to variation treatment with nitrosoguanidine to provide a variant BR bacterium having high productivity of alkaline protease (APE). The bacterial cells of the BR are then subjected to extraction treatment by a lysozyme treating method to afford a genetic DNA, having a promoter region with ability to express the APE and consisting of a base sequence expressed by the formula. The resultant DNA is subsequently linked to a vector plasmid pBR322 to afford a recombinant plasmid, which is used to transform a bacterium of the genus Bacillus (e.g. Bacillus subtilis) by a protoplast method. The obtained transformant strain is then cultivated in a culture medium containing a carbon source and nitrogen source under aerobic condition by adjusting to a desired pH and temperature to produce and accumulate APE in the culture fluid. The resultant APE is subsequently extracted, purified and recovered. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5543322-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9725704-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2666561-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H03285683-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0519229-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015505463-A |
| priorityDate | 1987-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.