http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S63214182-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_527cbadc3d8cf81b5bf931c0dd99b3c1 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 |
filingDate | 1987-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a26ac95fa7837a15f08dd477f35deefd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6c37eb0bfa7a2d2d009c17fbaee390c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8905d0c684fbc292295ccdb54673309d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_194546c6daaeaede2d0f915b9e5581b0 |
publicationDate | 1988-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-S63214182-A |
titleOfInvention | Production of heat-resistant isocitric acid dehydrogenase |
abstract | PURPOSE:To enable the mass production of the title enzyme excellent in heat resistance, mutation resistance and stability by culturing a microorganism in Thermus or Sulfolobus, producing heat-resistant isocitric acid dehydrogenase. CONSTITUTION:For example, a microorganism producing heat-resistant isocitric acid dehydrogenase in Thermus or Sulfolobus, which has been separated from a hot spring is cultured at elevated temperature such as 65-85 deg.C in a usual culture medium for bacteria under neutral for acidic conditions. Then, the product is treated by, e.g., centrifugation or filtration and the fractions rich in the title enzyme is, when necessary, subjected to contaminant proteins, then separated and purified to give the title enzyme of high purity and high activity. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0969099-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5871948-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0750046-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6352847-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0969099-A3 |
priorityDate | 1987-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.