| abstract |
(57) Summary The present invention provides that bacteria of this genus generally detect the presence of β-glycosidase produced by other bacteria, even though staphylococci produce the enzyme β-glucosidase. Experiments first reported herein that are unable to produce metabolites that would enzymatically react with the substrate used, 5-bromo-4-chloro-3-indolyl-β-D-glucopyranoside. Use the results of static observation. In this unexpected observation, one embodiment of the present invention provides an inhibitor for promoting the growth of Staphylococcus, as well as a second such as 5-bromo-4-chloro-3-indolyl-β-D-glucopyranoside. Includes a selective solid medium containing one glucopyranoside substrate and a second phosphatase substrate such as 6-chloro-3-indolyl phosphate or 5-bromo-6-chloro-3-indolyl phosphate. In this embodiment, the staphylococci in the sample produce metabolites that will react with the phosphatase substrate in the medium to form colonies with red to magenta, while other bacteria in the sample are: It will produce β-glucosidase that will react with the glucopyranosidase substrate in the medium and will form colonies with a blue color. In a particularly preferred embodiment of the present invention, a dry culture medium comprising a selective inhibitor, a first glucopyranoside substrate, and a second phosphatase substrate is used to prepare a thin film culture plate device such as the PETRIFILM culture plate device. Upon incubating the sample in the thin film culture plate device for a sufficient period of time after incubating, the staphylococci in the sample form colonies with red color on the thin film device and other bacteria in the sample give blue color on the device. Will form colonies with. |