abstract |
(57) Abstract: 3-hydroxybutyrate unit (3HB Component) and 4-hydroxybutyrate unit (4HB component) In the method for producing the copolymer, which comprises a step of extracting and separating the hydroxyalkanoic acid copolymer accumulated in the cells of a microorganism having the ability to produce a hydroxyalkanoic acid copolymer, A method for producing, characterized in that extraction is carried out by mixing wet bacterial cells of the bacterial cells with acetone containing a surfactant and heating. [Effect] Since the copolymer can be extracted from the wet cells, it is not necessary to dry the cells, and the extraction time can be shortened, so that the efficiency of the production process can be improved. Furthermore, in the case where a copolymer having a high content of 3HB component and a copolymer having a high content of 4HB component are accumulated in the bacterial cells, the copolymer having a high content of 4HB component can be easily and accurately selectively separated and purified. can do. |