| abstract |
(57) [Abstract] [PROBLEMS] To express a target protein in a bacterial cell without insolubilizing it. SOLUTION: The sequence has a sequence in which a gene encoding a molecular chaperone is operably linked to a first promoter and a second promoter, and the gene encoding the protein of interest is inserted downstream of the second promoter. By transforming with the expression vector, the molecular chaperone and the target protein are simultaneously expressed, and the target protein is produced in the bacterial cells without insolubilizing it. |