http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H03187388-A

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filingDate 1989-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b42f14e26da72296f5b18755e5148a6
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publicationDate 1991-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H03187388-A
titleOfInvention Production of theanine by plant cell culture
abstract PURPOSE: To obtain theanine with industrial advantage while reducing the content of caffeine or tannin by carrying out cell culture of tea using a basal medium containing ethylamine and derivatives thereof. n CONSTITUTION: A slice of stem of tea sprout is subjected to tissue culture in a basal medium containing 1-250 mM ethylamine, derivatives thereof (single substance such as ethylamine hydrogenchloride or ethylamine hydrogensulfate or mixture thereof), an ammonical nitrogen and a nitrate nitrogen in an amount larger than that of the ammoniacal nitrogen as N source under illumination at 10-32°C so as to induce callus. The resultant callus is subjected to subculture in the above-mentioned basal medium (MS-TEA medium) and to further subculture under dark. The subcultured callus is then subjected to shake culture under dark in the above mentioned basal medium, thus giving the objective theanine. n COPYRIGHT: (C)1991,JPO&Japio
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105104194-A
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priorityDate 1989-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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