| abstract |
PURPOSE: To obtain a new D-amino acid amide hydrolase having substrate specificity and specific molecular weight from a culture mixture by culturing a bacterium belonging to the genus Achromobacter, capable of hydrolyzing amino acid amide to give the culture mixture. n CONSTITUTION: A bacterium [e.g. new strain, Achromobacter sp. SCRC-SV3 (FERM P-1060)], capable of hydrolyzing amino acid amide, is cultured. In the culture, preferably a liquid medium containing a nitrogen source such as ammonium sulfate, a carbon source such as starch and an inorganic salt such as K 2 HPO 4 or NaCl is used as the medium. The bacterium is cultured under an aerobic condition by shaking culture, aerated spinner culture, etc. The culture temperature is preferably 25-45°C and pH is 5-11. A D-amino acid amide hydrolase is collected from the prepared culture mixture and purified by using ordinary enzyme purification method. The enzyme is treated with D-amino acid amide or a D-amino acid amide-containing substance to form a D-amino acid. n COPYRIGHT: (C)1990,JPO&Japio |