http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H02231565-A

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
filingDate 1989-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9831a40a0d6b6b610a7d81b3a115924
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a605753a850baea4d35cb6c98ad06bd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7be28f83a1b04a365329f8344681abff
publicationDate 1990-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H02231565-A
titleOfInvention Reagent kit for measuring free hemoglobin and method for measuring free hemoglobin by using this kit
abstract PURPOSE: To directly and quantitatively measure free hemoglobin with a high sensitivity by using the solid phase human haptoglobin formed by conjugating human haptoglobin with a solid phase and the oxygen labeled antibody formed by conjugating oxygen with an anti-human haptoglobin antibody. n CONSTITUTION: A free hemoglobin-contg. specimen which is adjusted in concn. with a suitable buffer soln. at need is brought into reaction with the solid phase human haptoglobin. The supernatant is thereafter removed, by which the free hemoglobin conjugated specifically with the solid phase human haptoglobin is made to remain on the solid phase. The oxygen labeled antibody is then added thereto, by which the oxygen labeled antibody is conjugated with the free hemoglobin remaining on the solid phase. After the excess oxygen labeled antibody failing to be conjugated is removed, the oxygen activity is measured. On the other hand, the relation between the free hemoglobin and the oxygen activity is formed by making the similar operation on the sample solution of the standard free hemoglobin diluted to 3-5 stages. The free hemoglobin quantity in the specimen is determined by contrasting the resulted oxygen activity and a calibration curve. n COPYRIGHT: (C)1990,JPO&Japio
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2020066722-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3859338-A4
priorityDate 1989-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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