http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H02227077-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_746d8243701ae4271b74368451625bee |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 1989-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05a4aa5e66c8662bb8912c776b7282d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c056734e37d1e41591f61b85d48f6a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84d50dc4a41091730931166cdd75c019 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fe29ec87f56a2c8ec1029e783d3587d |
| publicationDate | 1990-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H02227077-A |
| titleOfInvention | Immobilized enzyme and utilizing method of the enzyme |
| abstract | PURPOSE: To preserve and manifest high activity and further produce polynucleotide in high yield at a low cost by using porous chitosan beads effectively immobilized with polynucleotide phosphorylase. n CONSTITUTION: Porous chitosan beads having 0.5-3.0mm particle diameter are immersed in water or buffer solution such as phosphoric acid and polynucleotide phosphorylase (PNPase) solution derived from fungus cell of microorganism such as Micrococcus luteus is added to said solution, then shaken at room temperature to adsorb and immobilize PNPase on the carrier beads. Said method is more advantageous than pretreatment of chitosan beads with crosslinking agent such as glutaraldehyde. Next, resultant immobilized enzyme is charged in a column and nucleoside such as nucleotide-5'-biphosphoric acid as substrate is continuously fed through the column to obtain polynucleotide. By said method, polynucleotide in high quality is able to be synthesized in high yield. n COPYRIGHT: (C)1990,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014088087-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011055719-A |
| priorityDate | 1988-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 860.