http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0195785-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cff62ee19588f053fe1f229df4e6c756 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-77 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-13 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1987-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1792969543b354af5d23ffa1834d69db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb81137f45570995b646b1402618f646 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26f5393ff772939a9813285ec00f34db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4029ff12dd76142add76207b4097808f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f02dabe8b1027e7a55a99c29837908a |
| publicationDate | 1989-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H0195785-A |
| titleOfInvention | Plasmid pby503 |
| abstract | PURPOSE:To facilitate autonomous propagation using a plasmid pBY503, by synthesizing the plasmid pBY503 having 10 megadaltons molecular weight and the numbers of cleavage sites by restriction enzymes as follows. 4; EcoRI, 1; BamHI, etc. CONSTITUTION:Microbial cells of Brevibacterium.stationis are subjected to treatment with a lysozyme to carry out bacteriolysis. The resultant bacteriolytic solution is then subjected to treatment with phenol chloroform. A plasmid is subsequently recovered from the treated solution into fractions by precipitation with ethanol and isolated by equilibrium density-gradient centrifugation with cesium chloride containing ethidium bromide. The isolated plasmid pBY503 has about 10 megadaltons molecular weight and the numbers of cleavage sites by restriction enzymes as follows. 4; EcoRI, 1; BamHI, 2; SauI, 3; KpnI and 2; SalI. This plasmid is useful as a vector for recombining genes. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7365807-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11239608-B2 |
| priorityDate | 1987-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.