| abstract |
A chimeric Factor VIII polypeptide and uses thereof. The present invention relates to a VWF fragment containing a D'domain and a D3 domain of VWF, a chimeric protein containing the VWF fragment and a heterologous portion, or a chimeric protein containing the VWF fragment and an FVIII protein, and a method of using the same. I will provide a. The polypeptide chain containing the VWF fragment of the present invention binds or associates with the polypeptide chain containing the FVIII protein, and the polypeptide chain containing the VWF fragment can prevent or inhibit the binding of the endogenous VWF to the FVIII protein. By preventing or inhibiting the binding of endogenous VWF, which is a half-life limiting factor of FVIII, to FVIII, the VWF fragment can induce a half-life extension of the FVIII protein. The invention also includes nucleotides, vectors, host cells, methods using VWF fragments or chimeric proteins. [Selection diagram] None |