http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020007309-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_79e22d2d6420ad7f7e788a6828ec2d27 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6419819278c4e69622dff8f635023155 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
filingDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a8f0cc28e884d6bb81c67b4404579145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d350d3abf9391a2ee6d5388258139cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c0701f4e9417b835f5f9c724fcc2c66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e14c4f6f687ad0cc3628a48f2c5d95c5 |
publicationDate | 2020-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2020007309-A |
titleOfInvention | Method for producing chemically cross-linked peptide, chemically cross-linked peptide prepared using the method, and peptide library constructed by using the peptide by cDNA display method |
abstract | The present invention provides a chemically crosslinked peptide having a stable structure under reducing conditions. An mRNA is prepared by transcription from a DNA having a desired sequence, and the mRNA is ligated to an mRNA binding site of a linker having a peptide binding site, a solid phase binding site and an mRNA binding site to prepare a linker, The linker is translated by a cell-free translation system, and a peptide corresponding to the mRNA is synthesized in a state of being bound to a peptide binding site of the linker, and the peptide-bonded linker is immobilized on a solid phase, and immobilized on the solid phase. By reducing the S—S bond in the peptide bonded to the linker thus formed by reduction, and then bonding the selected divalent cross-linking compound that forms a cross-link with the reduced —SH group A chemically cross-linked peptide having a cross-linking efficiency of at least 90% with a linker by gel shift assay in which an intramolecular cross-link has been formed. [Selection diagram] None |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021148870-A1 |
priorityDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 304.