| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-35
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-96466
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-553
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1203
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56955
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56911
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5375
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-557
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6857
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| filingDate |
2016-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2018-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2018538534-A |
| titleOfInvention |
Method for directly measuring affinity of human IgG1 binding to multimeric antigen |
| abstract |
We report a method for determining the binding affinity of the binding sites of bivalent full-length antibodies of the human IgG1 subclass to homomultimeric antigens. The method comprises the steps of i) mixing a mixture comprising said antibody and a polypeptide derived from porphyromonas gingivalis lysine gingivaline to cleave the antibody into Fab and Fc regions, pH 7.5 to pH 8.5. Incubating the reaction mixture obtained in the previous step at a temperature of 30 ° C. to 42 ° C. for a period of 10 minutes to 240 minutes in the presence of a reducing agent, and ii) subjecting the incubated reaction mixture obtained in the previous step to surface plasmon resonance Determining the binding affinity of the antibody Fab to its antigen using surface plasmon resonance by direct application, thereby determining the binding affinity of the binding site of the bivalent full-length antibody of the human IgG1 subclass including. |
| priorityDate |
2015-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |