| abstract |
A composition comprising at least one protein component of RNA-derived endonuclease (RGEN) and at least one cell penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non- Disclosed are compositions that are covalently linked together to form a RGEN protein-CPP complex. The RGEN protein-CPP complex can cross (i) the cell membrane, or (ii) the cell wall and cell membrane of the cell. In certain embodiments, the RGEN protein component of the RGEN protein-CPP complex can be combined with an appropriate RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed is a composition comprising at least one protein component of a guide polynucleotide / Cas endonuclease complex, and at least one CPP, a method for transporting RGEN protein into microbial cells, and a method for targeting DNA with RGEN. . |