| abstract |
This disclosure provides systems and methods for attaching nanopore detectable tags to nucleotides. The present disclosure also provides a method for sequencing a nucleic acid using the disclosed tagged nucleotides. Provided herein are nucleotides with attached tags and methods for attaching tags to nucleotides. Tags can be attached by chemical reactions such as “click chemistry”. In one aspect, the disclosure provides (a) a polyphosphate group having a terminal phosphate, and (b) a triazole, 1,2-diazine, disulfide, secondary amine, hydrazone, thioacetamide, or maleimide-thio addition. The body provides a tagged nucleotide comprising a tag covalently linked to the terminal phosphate of the nucleotide. [Selection] Figure 1 |