| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad2cd796f8c4dcb425ddcc68707d3444 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2016-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb941338fef44ea1de67957aa952dec7 |
| publicationDate |
2017-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2017209036-A |
| titleOfInvention |
Improved virus detection method |
| abstract |
A method and kit that enable detection of the presence or absence of viral RNA by one-step RT-PCR in addition to a reaction solution without isolation of non-enveloped virus RNA from the sample or prior heat treatment of the sample provide. A method for testing an RNA virus having no envelope in a sample, for testing the presence or absence of a virus comprising the following steps without RNA purification from the sample or prior heat treatment of the sample: Method. (1) A step of mixing a sample and a reagent containing a chaotropic agent, (2) adding a one-step RT-PCR reaction solution containing a reverse transcriptase and a thermostable DNA polymerase to the mixed solution; (3) A step of carrying out a one-step RT-PCR reaction after sealing the reaction vessel. [Selection figure] None |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020080806-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018198682-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2021010239-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022210122-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021200717-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022024934-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021221109-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111254218-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114375342-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021010239-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20200066164-A |
| priorityDate |
2016-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |