abstract |
The present invention further provides an improved 7β-HSDH, more particularly the preparation of enzymatic or microbial UDCA via stereoselective reduction of DHCA from position 7 to 3,12-diketo-7β-CA. Provision of enzyme mutants that can be used to A 7β-hydroxysteroid dehydrogenase (7β-HSDH) mutant that catalyzes a 7-ketosteroid to a 7-hydroxysteroid corresponding to the 7-ketosteroid by at least stereospecific enzymatic reduction, comprising: The variant has at least one mutation in the sequence motif VMVGGRRE at positions 36-42 of SEQ ID NO: 2 and has at least 90% sequence identity with SEQ ID NO: 2 due to the 7β-HSDH variant described above. Solve the problem. [Selection figure] None |