| abstract |
To provide a highly engrafted cultured periodontal ligament cell sheet having good adhesion to the root surface. [MEANS FOR SOLVING PROBLEMS] A cell is cultured under specific conditions on a cell culture support in which a substrate surface is coated with a temperature-responsive polymer having an upper or lower critical dissolution temperature in water of 0 to 80 ° C. The cultured periodontal ligament cell sheet produced by setting the liquid temperature to the upper critical solution temperature or lower or the lower critical solution temperature or lower, (2) adhering the cultured cultured periodontal ligament cell sheet to the carrier, and (3) peeling with the carrier as it is Has a ligament-like microstructure, has extremely high engraftment on the root surface, can be transplanted with target cells at a high density, and can actively rebuild periodontal tissue. Furthermore, by stacking the cell sheets to be transplanted and having a three-dimensional polarity, it is possible to reconstruct the attached organs more efficiently, such as moderate periodontitis, severe periodontitis, and gingival recession. Clinical application is strongly expected. [Selection figure] None |