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filingDate 2015-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8289258c301870fa2177bed78447365
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publicationDate 2015-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2015211688-A
titleOfInvention Method for inducing differentiation of embryonic stem cells or induced pluripotent stem cells
abstract Provided is a method for easily inducing differentiation of a large amount of embryonic stem cells or induced pluripotent stem cells using a substrate surface having a non-cell-adhesive region around a cell-adherent region. A substrate surface having a non-cell-adhesive region around a cell-adhesive region is used, (1) the cells are attached to the cell-adhesive region on the substrate surface under a differentiation-inducing medium, 2) Thereafter, the cells are grown until they become confluent on the cell adhesion surface, and (3) the culture is further continued, and the cells are three-dimensionally separated from the boundary with the cell non-adhesion region around the cell adhesion region. And (4) embryonic stem cells that combine a plurality of layered portions finally formed in the cell adhesion region to produce a thick cell aggregate in the entire cell adhesion region Alternatively, a method for inducing differentiation of induced pluripotent stem cells. [Selection figure] None
priorityDate 2009-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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