abstract |
Provided are constructs and libraries comprising antibody surrogate light chain sequences. B-cell progenitors (pre-B cells) produce μ heavy chains, but instead of fully developed light chains, B lineage-specific genes called VpreB (l-3) and λ5, respectively. It has been identified in the bone marrow as a lymphocyte expressing set. The present invention relates to constructs and libraries comprising antibody surrogate light chain sequences. In particular, the invention relates to a construct comprising a VpreB sequence optionally partnered with another polypeptide, such as, for example, an antibody heavy chain variable domain sequence, and a library containing it. [Selection figure] None |