abstract |
The expression level of a desired gene is greatly improved when a filamentous fungus is used as a host cell. A cis-acting element according to the present invention has a region in which an XlnR / Ace2 binding sequence (ggctaa) and a Hap complex binding sequence (ccaat) are arranged via a spacer sequence of 0-100. Moreover, expression of a desired gene can be significantly improved by culturing a transformant having a cis-acting element according to the present invention in, for example, a xylan-containing medium. [Selection figure] None |