http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010263812-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c5b9bf80bd478ffae8859e807e755b3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2009-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b58f2e7b16553151952d9b583b2d4db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc4629ef55b70efdeccf4ae316a48d67 |
| publicationDate | 2010-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2010263812-A |
| titleOfInvention | Method for producing crustacean nuclease |
| abstract | The present invention provides a method for efficiently and efficiently producing a crustacean-derived nucleolytic enzyme having a soluble and enzymatic activity by a recombinant technique. The host is infected with a recombinant vector incorporating a gene encoding a crustacean-derived nucleolytic enzyme, and the incubation of the host for the production of the enzyme is carried out for 8-12 times higher than the optimal growth temperature of the host. Perform at a lower temperature. By using a signal peptide sequence in which degradation or inactivation of the enzyme in the host is reduced and the enzyme is not secreted and dissipated out of the host, the enzyme accumulates in the host at a high concentration and is treated with protease. Can be recovered efficiently. [Selection] Figure 7 |
| priorityDate | 2009-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 130.