http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010178716-A

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filingDate 2009-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4a7a98e61904638a4c39b80017685ed
publicationDate 2010-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2010178716-A
titleOfInvention Target nucleic acid molecule quantification method and target nucleic acid molecule quantification kit
abstract Provided is a method for accurately quantifying nucleic acid molecules present in a sample with high sensitivity. (A) Complementary to a nucleic acid-containing sample, a first nucleic acid molecule probe having a base sequence complementary to a target nucleic acid molecule to which a first marker is bound, and a first nucleic acid molecule probe to which a second marker is bound A step of preparing a sample solution to which a second nucleic acid molecule probe having a specific base sequence is added; (b) a step of denatured nucleic acid molecules in the sample solution prepared in step (a) and then associating; (C) after step (b), quantifying the target nucleic acid molecule by detecting temporal changes in the optical properties of the first marker or the second marker in the sample solution, A nucleus in which at least one of the marker and the second marker is a substance whose optical properties change depending on whether or not the first nucleic acid molecule probe and the second nucleic acid molecule probe are associated. The method of quantifying a target nucleic acid molecule containing the sample. [Selection figure] None
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