http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009543582-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2008-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2009543582-A |
| titleOfInvention | Generalized methods-high-throughput mutation screening methods and kits using sequential adapter ligation and amplification enabled by specific sequence complementation (DSF) |
| abstract | The present disclosure teaches a high-throughput mutation screening that enables simultaneous analysis of diverse gene regions and sensitive detection of very infrequent mutations in a generalized manner. The method can be achieved by treatment of RNA: DNA heteroduplex with ribonuclease, sequence extension with DNA polymerase using RNA primer, ligation with blocking adapter, and single strand specific nuclease after specific sequence complementation Full length sequence extension to become, and only the mutant complemented by complementary deoxyribonucleotide triphosphates followed by ligation with a labeled reporter adapter. By forming a labeled mutant-double-stranded adapter hybrid or mutant-three-stranded adapter hybrid, the detection and / or quantification of the mutant can be performed regardless of the source or the number of mutations involved. Aimed at shared labeling or flanking adapter sequences to amplify / amplify sequences in all variants, thereby avoiding the enormous effort involved in amplifying various target-specific sequences. The method is carried out in whole or in part on a solid medium in liquid form and, on a large scale, can be adapted to automated or semi-automated analysis and combinations thereof. [Selection figure] None |
| priorityDate | 2007-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 122.