http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009523740-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-182 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-02005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1014 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D475-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12 |
| filingDate | 2007-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2009523740-A |
| titleOfInvention | Method for regioselective N (5) -formylation of (6S) -5,6,7,8-tetrahydropteroic acid and its derivatives |
| abstract | The method of the present invention for regioselectively N (5) -formylating (6S) -5,6,7,8-tetrahydropteroic acid and its derivatives represented by Formula I is 6.5-7. An aqueous buffer having a pH of 5 is added to the mixture of the compound of formula I, antioxidant and formyl donor in a reaction vessel, and an inert gas is blown into the resulting suspension, An aqueous solution is added dropwise or in several portions with continuous stirring to obtain a transparent solution having a pH of 6.5 to 8.3, and the enzyme is a recombinant monofunctional glutamate formyltransferase (rGFT) Is added to the aqueous buffer and the temperature is increased to 30 ° C. to 45 ° C. to react the reaction mixture, resulting in at least 95% of the transfer of formyl from the formyl donor to the compound of formula I. Let and get The reaction mixture is cooled to 0 ° C. to 5 ° C., and an inorganic acid aqueous solution is dropped to adjust the pH to 2.7 to 3.1, thereby precipitating the unpurified compound represented by Formula II and completing the precipitation. For this purpose, stirring is continued for at least 1 hour to obtain an unpurified compound of formula II. |
| priorityDate | 2006-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 90.