| abstract |
A liquid reagent composed of two or more reagents, in the presence of an electron acceptor, an enzyme having an oxidizing ability by dehydrogenation is allowed to act on a biological component, and the reduced form of the electron acceptor produced is an electron To achieve both high precision and stability of the reagent in the method of forming and quantifying formazan by acting a carrier and a reducing dye. A method in which tetrazolium or a compound thereof is used as a reducing dye, the reagent containing the reducing dye has a pH of 8.0 or less, and the reagent has a pH of 7.5 or more during the reaction. [Selection figure] None |