http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009008608-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af92759c928daeb7f0daabbb0499f059 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6872 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-536 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2007-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e78b4b74731e67376478982f3e1ad44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a046c1163cd50bd066a12254b5f2ff5 |
| publicationDate | 2009-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2009008608-A |
| titleOfInvention | Method for quantifying DNA-binding protein and kit for quantification |
| abstract | PROBLEM TO BE SOLVED: To provide a method capable of quantifying the content of a target DNA binding protein in a liquid sample such as a biological sample containing impurities with high accuracy in a short time without performing separation and purification operation, and a quantification kit therefor provide. In a mixed solution A in which a liquid sample is mixed with a reagent A containing a fluorescently labeled nucleic acid probe Pfw having a nucleic acid sequence to which a target DNA binding protein binds and an unlabeled nucleic acid probe Pw having the nucleic acid sequence. A translational diffusion time Ta; and a translational diffusion time in a mixed solution B in which the liquid sample is mixed with a reagent B containing an unlabeled nucleic acid probe Pn composed of a nucleic acid sequence to which the fluorescently labeled nucleic acid probe Pfw and the DNA-binding protein do not bind. Tb is obtained by fluorescence correlation spectroscopy, and the amount of the target DNA-binding protein in the sample is obtained based on the difference between the translational diffusion times | Ta−Tb |. The quantification kit is a combination of a reagent A containing probes Pfw and Pw and a reagent B containing probes Pfw and Pn. [Selection] Figure 2 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015146813-A |
| priorityDate | 2007-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.