| abstract |
(57) Abstract: A method for quantifying a DNA-binding protein in a biological sample. A method for quantifying a DNA-binding protein that interacts with DNA in a biological sample with a higher sensitivity than an electrophoretic mobility shift assay (EMSA) without using a radioisotope, Treating the sample to obtain a liquid to be tested for the DNA binding protein, incubating the liquid containing the DNA binding protein with DNA containing a binding site to which the DNA binding protein can specifically bind, Specifically binds to DNA, and DNA with the bound DNA binding protein Is separated from other DNA and proteins in the liquid, And detecting the separated DNA binding protein using a test that produces a first numerical result proportional to the amount of the separated DNA binding protein. |